SKU: 17005880873

Sheep AP13 (Apelin 13) ELISA Kit

Sale price$202.50 Regular price$225.00
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Ships within 48 hours · Estimated delivery Jul 14 - Jul 19

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Description

Sheep AP13 (Apelin 13) ELISA KitThe Sheep AP13 (Apelin 13) ELISA immunoassay kit uses the Competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with Sheep AP13. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin conjugated antibody specific to Sheep AP13. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The

The Sheep AP13 (Apelin 13) ELISA immunoassay kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Sheep AP13. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to Sheep AP13. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep AP13 in the samples is then determined by comparing the OD of the samples to the standard curve.

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SKU: 17005880873

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4.8 ★★★★★
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Grumpy
Belleville, US
★★★★★ 5
This works better than any other product tried
This is the best. I had tried hemp chews, chamomile and lavender ointment, and thunder vest. And at times all three for my dogs anxiety during storms and any loud noise. Nothing worked. Got this and it calmed him almost immediately and actually calmed him so much he just fell asleep. Glad I ordered two. One for upstairs and down. It’s very cute and soft. Great product with a great price. It’s holding up good so far. Only had for a couple of weeks. Hope it holds up for awhile. As he is not a chewer.
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Reviewed in the United States on July 28, 2025
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Libs
Los Angeles, US
★★★★★ 5
Great product. So cute
So pleased. Helped my 11 week puppy sleep in his bed without crying. Would like smaller ones for Yorkies.
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Reviewed in the United States on October 13, 2025
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Leyda
Omaha, US
★★★★★ 5
Its amazing
My dog ​​calms down when I put her plush toy near her; when I hear the sound of its heartbeat, I know she's very still.
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Reviewed in the United States on March 6, 2026
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Tonya
Phoenix, US
★★★★★ 2
Dogs can open zipper and pull out all the stuffing easily
Not great for a chewer. My 15 week puppy unzipped it and pulled all of the stuffing out. The stuffing is just loose once you unzip it, there isnt a cloth pocket for the battery pack. She also ate the rectangle hand hold part of the zipper. Really sad this didn't work out. I do feel like it helped her settle a little but just wasn't safe for her. Other than that, it appeared as pictured and worked properly. Also worth noting, the heart beat is LOUD when you put batteries in. I don't know if it calms over time but it was kind of intense.
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Reviewed in the United States on June 7, 2025
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CT9
Natrona Heights, US
★★★★★ 3
Zipper is a problem
The first set we received the zipper separated. They were quickly replaced. The zipper tab is very large and our puppy is able to pull it down and pull out all the stuffing. Otherwise it worked to help comfort her until she found the zipper.
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Reviewed in the United States on March 20, 2025

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